Our Human Primary-Like Pancreatic Beta Cell-Based assays provide a high-throughput physiologically relevant platform for measuring glucose-stimulated insulin secretion (GSIS) while enabling assessment of GIPR, GLP1R, and GCGR receptor activity through cAMP accumulation assays. Together, these assays generate rich mechanistic insights, including potency, efficacy, signaling bias, and pathway engagement, helping to bridge early discovery and translational research while accelerating the development of next-generation metabolic therapies.
Why Researchers Choose GSISscan
RELEVANCE:
- Physiologically relevant functional endpoint with endogenous receptor expression levels
- Evaluates glucose dependent insulin secretion in the presence and absence of incretins
- Concurrent incretin receptor activation with cAMP assay
- Supports incretin drug development
CHALLENGE:
- Lack of high-throughput in vitro assays for rapid GSIS measurements
- Current options include in vivo models with complicated surgeries or ex vivo assays with isolated pancreatic islet cells
- Both of these are time-consuming and resource-intensive
VALUE:
- Primary-Like Cells
- Co-expression of functional GIPR, GLP1R and GCGR
- Evaluate basal and incretin-enhanced glucose-stimulated insulin secretion (GSIS)
- Measure insulin and cAMP with human pancreatic β-cells in two separate assays
- Characterize proximal receptor activation and functional insulin secretion within one biological system
- High-throughput, automation-compatible in vitro workflow
- Robust assay performance and reproducibility
- 10-point (3-fold) dose response curves in singlicate
ASSAY PORTFOLIO:
| Item Number | Item Name | Description | Key Features | Applications |
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| 86-0037P-0001AG | Human Primary-like Pancreatic Beta Cell-Based Agonist Glucose-Stimulated Insulin Secretion GSISscan LeadHunter Assay – US | Measures glucose-stimulated insulin secretion from human primary-like pancreatic β-cells, enabling direct evaluation of β-cell responsiveness and insulinotropic compounds |
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| 86-0037P-0002AG | Human Primary-like Pancreatic Beta Cell-Based Agonist cAMP (TR-FRET) LeadHunter Assay – US | Measures cAMP accumulation in human β-cells, providing mechanistic insight into Gs-coupled GPCR activity, including key incretin receptors like GLP-1 (GLP1R), Glucagon (GCGR) and GIP (GIPR) receptors |
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| 86-0037P-0002AN | Human Primary-like Pancreatic Beta Cell-Based Antagonist cAMP (TR-FRET) LeadHunter Assay – US |
