High-throughput Measurement of Aggregation

Aggregation is a serious concern with large molecule therapeutics. Aggregated molecules are highly immunogenic, meaning that they cause aberrant activation of the immune system. This can result in drug reactions, side effects, and ultimately make a medication unsafe for administration.
 
Concentrating samples to a therapeutic dose can cause molecules to destabilize and clump or aggregate together. Conditions required for administration, such as buffer, temperature, transport, and storage can cause large molecules such as proteins to unfold and stick together or aggregate. Optimization of leads for manufacturing often requires a combination of modifications to both drug and buffer conditions to ensure stability across a wide range of conditions that would be encountered in the lifetime of a drug sample.
 
At Eurofins Discovery, we can perform high-throughput, small-scale assays for aggregation. This testing can occur in combination with drug concentration, buffer exchange, and forced degradation testing.
 
Our expansive technical capabilities include instrumentation that can perform measurements on as little as 2 microliters of sample material — increasing the number of conditions and time points that can be assessed and maximizing your available material.